Identification polymorphism of LHR and FSHR genes in Indonesian Holstein dairy cattle associated with productive and reproductive traits

Abstract. Setyorini YW, Kurnianto E, Sutopo, Sutiyono. 2023. Identification polymorphism of LHR and FSHR genes in Indonesian Holstein dairy cattle associated with productive reproductive traits. Biodiversitas 24: 2898-2905. Luteinizing follicle-stimulating hormones their receptors play an important role the system’s hormonal activity physiological function. This study aimed to identify genotype variation Hormone Receptor (LHR) gene exon 11 Follicle-stimulating (FSHR) 10 elucidate associating between milk traits cattle. A total 100 samples deoxyribonucleic acid (DNA) from were used this study. Polymerase Chain Reaction Restriction Fragment Length Polymorphism (PCR-RFLP) was performed for genotyping Single Nucleotide (SNP) HhaI AluI restriction enzymes, respectively. The collected phenotype data production, quality, trait. In addition, general linear model procedure SAS program carried out investigate association genotypes results showed that population monomorphic, while polymorphic three genotypes, namely CC, CG, GG. sequences indicated presence a mutation at nucleotide number 2037 substitutes cytosine guanine. Cows CC better parameter service per conception (P<0.05) than other but did not correlate parameters Therefore, it concluded SNP g.2037C>G identified Dairy